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產(chǎn)品詳情
  • 產(chǎn)品名稱:重組大鼠粒細胞 - 巨噬細胞集落刺激因子蛋白

  • 產(chǎn)品型號:rRtGM-CSF
  • 產(chǎn)品廠商:KALANG
  • 產(chǎn)品文檔:
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重組大鼠粒細胞 - 巨噬細胞集落刺激因子蛋白與其它公司提供的重組蛋白不同,rRtGM-CSF蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠粒細胞 - 巨噬細胞集落刺激因子蛋白(全長序列)產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組大鼠粒細胞 - 巨噬細胞集落刺激因子蛋白

Synonyms Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
Species Rat
Accession P48750
GeneID 116630
Source Escherichia coli.
Molecular Weight 重組大鼠粒細胞 - 巨噬細胞集落刺激因子蛋白Approximately 14.5 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK
Purity 重組大鼠粒細胞 - 巨噬細胞集落刺激因子蛋白> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine FDC-P1 is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 重組大鼠粒細胞 - 巨噬細胞集落刺激因子蛋白Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/μg of rRtGM-CSF as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠粒細胞 - 巨噬細胞集落刺激因子蛋白
Reference 1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.
2. 1989. N Engl J Med, 320: 253-4.
3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.
4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.
5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.
Background Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. It is reported that GM-CSF has no biological effects across species.

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