產(chǎn)品詳情
簡單介紹：
重組鼠腎成纖維細(xì)胞瘤過表達(dá)的基因蛋白同源蛋白與其它公司提供的重組蛋白不同，rMuNOV蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠腎成纖維細(xì)胞瘤過表達(dá)的基因蛋白同源蛋白（全長序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測、ELISA等試驗(yàn)中.
詳情介紹：
重組鼠腎成纖維細(xì)胞瘤過表達(dá)的基因蛋白同源蛋白
| Species | Murine |
| Accession | Q64299 |
| GeneID | 18133 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠腎成纖維細(xì)胞瘤過表達(dá)的基因蛋白同源蛋白Approximately 36.4 kDa, a single non-glycosylated polypeptide chain containing 333 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | QVSASLRCPS RCPPKCPSIS PTCAPGVRSV LDGCSCCPVC ARQRGESCSE MRPCDQSSGL YCDRSADPNN QTGICMVPEG DNCVFDGVIY RNGEKFEPNC QYFCTCRDGQ IGCLPRCQLD VLLPGPDCPA PRKVAVPGEC CEKWTCGSDE QGTQGTLGGL ALPAYRPEAT VGVEVSDSSI NCIEQTTEWS ACSKSCGMGV STRVTNRNRQ CEMVKQTRLC IVRPCEQEPE EVTDKKGKKC LRTKKSLKAI HLQFENCTSL YTYKPRFCGV CSDGRCCTPH NTKTIQVEFQ CLPGEIIKKP VMVIGTCTCY SNCPQNNEAF LQDLELKTSR GEI |
| Purity | 重組鼠腎成纖維細(xì)胞瘤過表達(dá)的基因蛋白同源蛋白> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl. |
| Endotoxin | Less than 0.1 EU/μg of rMuNOV as determined by LAL method. |
| Reconstitution | 重組鼠腎成纖維細(xì)胞瘤過表達(dá)的基因蛋白同源蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠腎成纖維細(xì)胞瘤過表達(dá)的基因蛋白同源蛋白 |
