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產(chǎn)品名稱：重組小鼠胰島素樣生長因子-1蛋白
產(chǎn)品型號：rMuIGF-1
產(chǎn)品廠商：KALANG
產(chǎn)品文檔：

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簡單介紹：

重組小鼠胰島素樣生長因子-1蛋白與其它公司提供的重組蛋白不同，rMuIGF-1蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組小鼠胰島素樣生長因子-1蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組小鼠胰島素樣生長因子-1蛋白

Species Murine

Accession P05017

GeneID 16000

Source Escherichia coli.

Molecular Weight 重組小鼠胰島素樣生長因子-1蛋白Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

Quantity 10μg/50μg/1000μg

AA Sequence GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組小鼠胰島素樣生長因子-1蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.

Endotoxin 重組小鼠胰島素樣生長因子-1蛋白Less than 0.1 EU/μg of rMuIGF-1 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組小鼠胰島素樣生長因子-1蛋白

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Species	Murine
Accession	P05017
GeneID	16000
Source	Escherichia coli.
Molecular Weight	重組小鼠胰島素樣生長因子-1蛋白Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
Quantity	10μg/50μg/1000μg
AA Sequence	GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組小鼠胰島素樣生長因子-1蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.
Endotoxin	重組小鼠胰島素樣生長因子-1蛋白Less than 0.1 EU/μg of rMuIGF-1 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組小鼠胰島素樣生長因子-1蛋白

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產(chǎn)品名稱：重組小鼠胰島素樣生長因子-1蛋白