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產品名稱：重組鼠血小板因子-4 / CXCL4蛋白
產品型號：rMuPF-4/CXCL4
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組鼠血小板因子-4 / CXCL4蛋白與其它公司提供的重組蛋白不同，rMuPF-4/CXCL4蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠血小板因子-4 / CXCL4蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組鼠血小板因子-4 / CXCL4蛋白

Species Murine

Accession Q9Z126

GeneID 56744

Source Escherichia coli.

Molecular Weight 重組鼠血小板因子-4 / CXCL4蛋白Approximately 8.2 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組鼠血小板因子-4 / CXCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4.

Endotoxin Less than 1 EU/μg of rMuPF-4/CXCL4 as determined by LAL method.

Reconstitution 重組鼠血小板因子-4 / CXCL4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組鼠血小板因子-4 / CXCL4蛋白

Reference 1. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
2. Eisman R, Surrey S, Ramachandran B, et al. 1990. Blood. 76:336-44.
3. Lasagni L, Francalanci M, Annunziato F, et al. 2003. J Exp Med. 197:1537-49.
4. Warkentin TE. 2007. N Engl J Med. 356:891-3.

Background CXCL4 (PF4) is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. Specifically, Human and mouse CXCL4 share about a 60 % identity.

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Species	Murine
Accession	Q9Z126
GeneID	56744
Source	Escherichia coli.
Molecular Weight	重組鼠血小板因子-4 / CXCL4蛋白Approximately 8.2 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組鼠血小板因子-4 / CXCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4.
Endotoxin	Less than 1 EU/μg of rMuPF-4/CXCL4 as determined by LAL method.
Reconstitution	重組鼠血小板因子-4 / CXCL4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠血小板因子-4 / CXCL4蛋白
Reference	1. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42. 2. Eisman R, Surrey S, Ramachandran B, et al. 1990. Blood. 76:336-44. 3. Lasagni L, Francalanci M, Annunziato F, et al. 2003. J Exp Med. 197:1537-49. 4. Warkentin TE. 2007. N Engl J Med. 356:891-3.
Background	CXCL4 (PF4) is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. Specifically, Human and mouse CXCL4 share about a 60 % identity.

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產品名稱：重組鼠血小板因子-4 / CXCL4蛋白