產(chǎn)品詳情
簡(jiǎn)單介紹：
重組鼠成纖維細(xì)胞生長(zhǎng)因子16蛋白與其它公司提供的重組蛋白不同，rMuFGF-16蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組鼠成纖維細(xì)胞生長(zhǎng)因子16蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組鼠成纖維細(xì)胞生長(zhǎng)因子16蛋白
| Species | Murine |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 23.8 kDa, a single non-glycosylated polypeptide chain containing 207 amino acids. |
| AA Sequence | 重組鼠成纖維細(xì)胞生長(zhǎng)因子16蛋白MAEVGGVFAS LDWDLHGFSS SLGNVPLADS PGFLNERLGQ IEGKLQRGSP TDFAHLKGIL RRRQLYCRTG FHLEIFPNGT VHGTRHDHSR FGILEFISLA VGLISIRGVD SGLYLGMNER GELYGSKKLT RECVFREQFE ENWYNTYAST LYKHSDSERQ YYVALNKDGS PREGYRTKRH QKFTHFLPRP VDPSKLPSMS RDLFRYR |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data not available. |
| Physical Appearance | Sterile colorless liquid. |
| Formulation | 重組鼠成纖維細(xì)胞生長(zhǎng)因子16蛋白Supplied as a 0.2 μm filtered solution in 20 mM Tris-HCl, pH 9.0, 1 M NaCl, 0.02 % Tween-20, 10 % Glycerol. |
| Endotoxin | Less than 0.1 EU/μg of rMuFGF-16 as determined by LAL method. |
| Storage | This preparation should be kept at -20 °C for long term storage. Avoid repeated freeze/thaw cycles. |
| 重組鼠成纖維細(xì)胞生長(zhǎng)因子16蛋白 | |
| Background | 重組鼠成纖維細(xì)胞生長(zhǎng)因子16蛋白Fibroblast growth factor 16 (FGF-16) belongs to the large FGF family. All FGF family members are heparin-binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-16 was originally identified in rat heart tissue by homology based polymerase chain reaction. Murine FGF-16 cDNA predicts a 207 aa precursor protein with one N-linked glycosylation site. FGF-16 lacks a typical signal peptide, but is efficiently generated by mechanisms other than the classical protein secretion pathway. Among FGF family members, FGF-16 is most similar to FGF-9, sharing 73% aa sequence homology. Murine FGF-16 shares 99.5% and 99% aa sequence identity with the human and rat FGF-16, respectively. |
