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產品名稱：重組人瘦蛋白蛋白
產品型號：rHuLeptin
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人瘦蛋白蛋白與其它公司提供的重組蛋白不同，rHuLeptin蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人瘦蛋白蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人瘦蛋白蛋白

Synonyms Obese Protein, Obesity Factor

Species Human

Accession P41159

GeneID 3952

Source Escherichia coli.

Molecular Weight 重組人瘦蛋白蛋白Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.

Quantity 200μg/1000μg/5000μg

AA Sequence VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人瘦蛋白蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin 重組人瘦蛋白蛋白Less than 1 EU/μg of rHuLeptin as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人瘦蛋白蛋白

SDS-PAGE

Reference 1. Li AJ, Wiater MF, Oostrom MT, et al. 2012. Am J Physiol Regul Integr Comp Physiol, 302: R1313-26.
2. Penicaud L, Meillon S, Brondel L. 2012. Biochimie, 94: 2069-74.
3. Kutlu S, Aydin M, Alcin E, et al. 2010. Brain Res, 1317: 87-91.
4. Kongmacheep P, Sirikulchayanonta C, Tungtrongchitr R, et al. 2009. J Med Assoc Thai, 92 Suppl 7: S108-14.

Background Human Leptin plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Ob (Lep) gene (Ob for obese, Lep for leptin) is located on chromosome 7 in humans. The protein is manufactured primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body. Leptin acts on receptors in the hypothalamus of the brain where it inhibits appetite by (1) counteracting the effects of neuropeptide Y (a potent feeding stimulant secreted by cells in the gut and in the hypothalamus); (2) counteracting the effects of anandamide (another potent feeding stimulant that binds to the same receptors as THC), and (3) promoting the synthesis of α-MSH, an appetite suppressant. This appetite inhibition is long-term, in contrast to the rapid inhibition of eating by cholecystokinin (CCK) and the slower suppression of hunger between meals mediated by PYY3-36. The absence of leptin (or its receptor) leads to uncontrolled food intake and resulting obesity.

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Synonyms	Obese Protein, Obesity Factor
Species	Human
Accession	P41159
GeneID	3952
Source	Escherichia coli.
Molecular Weight	重組人瘦蛋白蛋白Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
Quantity	200μg/1000μg/5000μg
AA Sequence	VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人瘦蛋白蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	重組人瘦蛋白蛋白Less than 1 EU/μg of rHuLeptin as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人瘦蛋白蛋白
SDS-PAGE
Reference	1. Li AJ, Wiater MF, Oostrom MT, et al. 2012. Am J Physiol Regul Integr Comp Physiol, 302: R1313-26. 2. Penicaud L, Meillon S, Brondel L. 2012. Biochimie, 94: 2069-74. 3. Kutlu S, Aydin M, Alcin E, et al. 2010. Brain Res, 1317: 87-91. 4. Kongmacheep P, Sirikulchayanonta C, Tungtrongchitr R, et al. 2009. J Med Assoc Thai, 92 Suppl 7: S108-14.
Background	Human Leptin plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Ob (Lep) gene (Ob for obese, Lep for leptin) is located on chromosome 7 in humans. The protein is manufactured primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body. Leptin acts on receptors in the hypothalamus of the brain where it inhibits appetite by (1) counteracting the effects of neuropeptide Y (a potent feeding stimulant secreted by cells in the gut and in the hypothalamus); (2) counteracting the effects of anandamide (another potent feeding stimulant that binds to the same receptors as THC), and (3) promoting the synthesis of α-MSH, an appetite suppressant. This appetite inhibition is long-term, in contrast to the rapid inhibition of eating by cholecystokinin (CCK) and the slower suppression of hunger between meals mediated by PYY3-36. The absence of leptin (or its receptor) leads to uncontrolled food intake and resulting obesity.

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產品名稱：重組人瘦蛋白蛋白