產(chǎn)品詳情
簡單介紹：
重組人Otoraplin蛋白與其它公司提供的重組蛋白不同，rHuOTOR蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人Otoraplin蛋白（全長序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測、ELISA等試驗(yàn)中.
詳情介紹：
重組人Otoraplin蛋白
| Synonyms | Fibrocyte-derived Protein, Melanoma Inhibitory Activity-like Protein |
| Species | Human |
| Source | Escherichia coli. |
| Molecular Weight | 重組人Otoraplin蛋白Approximately 12.7 kDa, a single non-glycosylated polypeptide chain containing 111 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | VHGIFMDRLA SKKLCADDEC VYTISLASAQ EDYNAPDCRF INVKKGQQIY VYSKLVKENG AGEFWAGSVY GDGQDEMGVV GYFPRNLVKE QRVYQEATKE VPTTDIDFFC E |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人Otoraplin蛋白Data Not Available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuOTOR as determined by LAL method. |
| Reconstitution | 重組人Otoraplin蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人Otoraplin蛋白 | |
| SDS-PAGE |
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| Reference | 1. Robertson NG, Heller S, Lin JS, et al. 2000. Genomics, 66: 242-8. |
| Background | OTOR also named Otoraplin and MIAL, is a 15 kDa disulfide bonded homodimer, which is secreted via the Golgi apparatus and is a member of the MIA/OTOR family. Members of this family which also includes MIA, MIA2, and TANGO share a Src homology-3 (SH3)-like domain. OTOR is mainly expressed in the cochlea of the inner-ear and appears to be involved in early chondrogenesis of the otic capsule, which is required for normal inner ear development and auditory function. The OTOR shares high sequence identity with the mouse (90%), chicken (80%), and bullfrog (60%) orthologs and with the related human CDRAP/MIA protein (43%). |
