產(chǎn)品詳情
簡單介紹：
重組人類胸腺基質(zhì)**細(xì)胞生成素蛋白與其它公司提供的重組蛋白不同，rHuTSLP蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類胸腺基質(zhì)**細(xì)胞生成素蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人類胸腺基質(zhì)**細(xì)胞生成素蛋白
| Species | Human |
| Accession | Q969D9 |
| GeneID | 85480 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人類胸腺基質(zhì)**細(xì)胞生成素蛋白Approximately 15.1 kDa, a single non-glycosylated polypeptide chain containing 132 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人類胸腺基質(zhì)**細(xì)胞生成素蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-7Rα and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuTSLP as determined by LAL method. |
| Reconstitution | 重組人類胸腺基質(zhì)**細(xì)胞生成素蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類胸腺基質(zhì)**細(xì)胞生成素蛋白 | |
| SDS-PAGE |
|
| Reference |
1. Osborn MJ, Ryan PL, Kirchhof N, et al. 2004. Blood, 103: 843-51. 2. Liu YJ, Soumelis V, Watanabe N, et al. 2007. Annu Rev Immunol, 25: 193-219. 3. Lu N, Wang YH, Arima K, et al. 2009. J Exp Med, 206: 2111-9. 4. Ziegler SF. 2010. Curr Opin Immunol, 22: 795-9. 5. Takai T. 2012. Allergol Int, 61: 3-17. |
| Background | Thymic stromal lymphopoietin (TSLP) is a hemopoietic protein belonging to the cytokine family and is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. It is mainly expressed in a number of tissues including heart, liver and prostate. TSLP signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. After binding STAT5 phosphorylation is induced resulting in the expression of downstream transcription factors. Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP has the functions of enhancing the maturation of CD11c+ dendritic cells and inducing allergic inflammation by directly activating mast cells. It expression is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, and eczema and other allergic states. But the factors inducing the activation of TSLP release are not clearly defined. Human TSLP shares approximately 43 % a.a. sequence identity with mouse TSLP. |
