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產(chǎn)品詳情
  • 產(chǎn)品名稱:重組人白細(xì)胞介素-36受體拮抗劑蛋白蛋白

  • 產(chǎn)品型號:rHuIL-36RA
  • 產(chǎn)品廠商:KALANG
  • 產(chǎn)品文檔:
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簡單介紹:
重組人白細(xì)胞介素-36受體拮抗劑蛋白蛋白與其它公司提供的重組蛋白不同,rHuIL-36RA蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白,可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細(xì)胞介素-36受體拮抗劑蛋白蛋白(全長序列)產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組人白細(xì)胞介素-36受體拮抗劑蛋白蛋白

Synonyms IL-1RP3, IL-1HY1, IL-1 delta, IL-1F5, IL-1ra homolog 1, IL-1L1
Species Human
Accession Q9UBH0
GeneID 26525
Source Escherichia coli.
Molecular Weight 重組人白細(xì)胞介素-36受體拮抗劑蛋白蛋白Approximately 16.8 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids.
Quantity 5μg/25μg/1000μg
AA Sequence VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD
Purity 重組人白細(xì)胞介素-36受體拮抗劑蛋白蛋白> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 20 ng/ml of recombinant human IL-36 beta.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 重組人白細(xì)胞介素-36受體拮抗劑蛋白蛋白Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/μg of rHuIL-36RA as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人白細(xì)胞介素-36受體拮抗劑蛋白蛋白
SDS-PAGE
Reference 1. Born TL, Smith DE, Garka KE, et al. 2000. J Biol Chem. 275:41528.
2. Dunn EF, Gay NJ, Bristow AF, et al. 2003. Biochemistry. 42:10938-44.
3. Barton JL, Herbst R, Bosisio D, et al. 2000. Eur J Immunol. 30:3299-308.
4. Debets R, Timans JC, Homey B, et al. 2001. J Immunol. 167:1440-6.
5. Towne JE, Renshaw BR, Douangpanya J, et al. 2011. J Biol Chem. 286:42594-602.
Background Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant human IL-36 RA contains 154 amino acid residues and it shares 91 % a.a. sequence identity with murine IL-36RA.

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