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產品名稱：重組人膠質細胞成熟因子beta蛋白
產品型號：rHuGMF-β
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人膠質細胞成熟因子beta蛋白與其它公司提供的重組蛋白不同，rHuGMF-β蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人膠質細胞成熟因子beta蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人膠質細胞成熟因子beta蛋白

Synonyms GMFB

Species Human

Accession P60983

GeneID 2764

Source Escherichia coli.

Molecular Weight 重組人膠質細胞成熟因子beta蛋白Approximately 16.6 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence SESLVVCDVA EDLVEKLRKF RFRKETNNAA IIMKIDKDKR LVVLDEELEG ISPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPVGCKPEQQ MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity Data Not Available.

Physical Appearance 重組人膠質細胞成熟因子beta蛋白Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuGMF-β as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組人膠質細胞成熟因子beta蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人膠質細胞成熟因子beta蛋白

Reference 1. Gandhi M, Smith BA, Bovellan M, et al. 2010. Curr Biol, 20: 861-7.
2. Roman AandTombarkiewicz B. 2009. Bioelectromagnetics, 30: 21-8.
3. Ohnishi T, Arita N, Hayakawa T, et al. 1993. Biochem Biophys Res Commun, 193: 518-25.
4. Nakano K, Kuwayama H, Kawasaki M, et al. 2010. Cytoskeleton (Hoboken), 67: 373-82.

Background The glia maturation factor beta belongs to the actin-binding proteins ADF family, GMF subfamily. It contains an ADF-H domain, but the research of crystallography and NMR reveals that there are structures different between human and mouse ADF-H domain. GMF-β is involved in the differentiation, maintenance, and regeneration of the nervous system. It also inhibition of proliferation of tumor cells.

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Synonyms	GMFB
Species	Human
Accession	P60983
GeneID	2764
Source	Escherichia coli.
Molecular Weight	重組人膠質細胞成熟因子beta蛋白Approximately 16.6 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	SESLVVCDVA EDLVEKLRKF RFRKETNNAA IIMKIDKDKR LVVLDEELEG ISPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPVGCKPEQQ MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	Data Not Available.
Physical Appearance	重組人膠質細胞成熟因子beta蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuGMF-β as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組人膠質細胞成熟因子beta蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人膠質細胞成熟因子beta蛋白
Reference	1. Gandhi M, Smith BA, Bovellan M, et al. 2010. Curr Biol, 20: 861-7. 2. Roman AandTombarkiewicz B. 2009. Bioelectromagnetics, 30: 21-8. 3. Ohnishi T, Arita N, Hayakawa T, et al. 1993. Biochem Biophys Res Commun, 193: 518-25. 4. Nakano K, Kuwayama H, Kawasaki M, et al. 2010. Cytoskeleton (Hoboken), 67: 373-82.
Background	The glia maturation factor beta belongs to the actin-binding proteins ADF family, GMF subfamily. It contains an ADF-H domain, but the research of crystallography and NMR reveals that there are structures different between human and mouse ADF-H domain. GMF-β is involved in the differentiation, maintenance, and regeneration of the nervous system. It also inhibition of proliferation of tumor cells.

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產品名稱：重組人膠質細胞成熟因子beta蛋白