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產品名稱：重組人I-TAC / CXCL11蛋白
產品型號：rHuI-TAC/CXCL11
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人I-TAC / CXCL11蛋白與其它公司提供的重組蛋白不同，rHuI-TAC/CXCL11蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人I-TAC / CXCL11蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人I-TAC / CXCL11蛋白

Synonyms Beta-R1, H174, IP-9, Small-inducible Cytokine B11

Species Human

Accession O14625

GeneID 6373

Source Escherichia coli.

Molecular Weight 重組人I-TAC / CXCL11蛋白Approximately 8.3 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人I-TAC / CXCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human IL-2 activated human T-lymphocytes is in a concentration range of 0.1-10 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuI-TAC/CXCL11 as determined by LAL method.

Reconstitution 重組人I-TAC / CXCL11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人I-TAC / CXCL11蛋白

SDS-PAGE

Reference 1. Tensen CP, Flier J, Rampersad SS, et al. 1999. Biochim Biophys Acta. 1446:167-72.
2. Cole KE, Strick CA, Paradis TJ, et al. 1998. J Exp Med. 187:2009-21.
3. Rani MR, Foster GR, Leung S, et al. 1996. J Biol Chem. 271:22878-84.
4. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22.

Background CXCL11 also known as I-TAC is belonging to the CXC chemokine family and shares 36 % and 37 % amino acid sequence homology with IP-10 and MIG, respectively. It is highly expressed in peripheral blood leukocytes, pancreas and liver. Expression of CXCL11 is strongly induced by IFN-γ and IFN-β, and weakly induced by IFN-α. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3, which with a higher affinity than do the other chemokines for this receptor, CXCL9 and CXCL10. Similar to CXCL10, CXCL11 has been shown to be a chemoattractant for IL-2-activated T-lymphocytes, but not for isolated T-cells, neutrophils or monocytes.

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Synonyms	Beta-R1, H174, IP-9, Small-inducible Cytokine B11
Species	Human
Accession	O14625
GeneID	6373
Source	Escherichia coli.
Molecular Weight	重組人I-TAC / CXCL11蛋白Approximately 8.3 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人I-TAC / CXCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human IL-2 activated human T-lymphocytes is in a concentration range of 0.1-10 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuI-TAC/CXCL11 as determined by LAL method.
Reconstitution	重組人I-TAC / CXCL11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人I-TAC / CXCL11蛋白
SDS-PAGE
Reference	1. Tensen CP, Flier J, Rampersad SS, et al. 1999. Biochim Biophys Acta. 1446:167-72. 2. Cole KE, Strick CA, Paradis TJ, et al. 1998. J Exp Med. 187:2009-21. 3. Rani MR, Foster GR, Leung S, et al. 1996. J Biol Chem. 271:22878-84. 4. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22.
Background	CXCL11 also known as I-TAC is belonging to the CXC chemokine family and shares 36 % and 37 % amino acid sequence homology with IP-10 and MIG, respectively. It is highly expressed in peripheral blood leukocytes, pancreas and liver. Expression of CXCL11 is strongly induced by IFN-γ and IFN-β, and weakly induced by IFN-α. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3, which with a higher affinity than do the other chemokines for this receptor, CXCL9 and CXCL10. Similar to CXCL10, CXCL11 has been shown to be a chemoattractant for IL-2-activated T-lymphocytes, but not for isolated T-cells, neutrophils or monocytes.

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產品名稱：重組人I-TAC / CXCL11蛋白