產品詳情
簡單介紹：
重組人骨形態發生蛋白7蛋白與其它公司提供的重組蛋白不同，rHuBMP-7蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人骨形態發生蛋白7蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人骨形態發生蛋白7蛋白
| Synonyms | OP-1 |
| Species | Human |
| Accession | P18075 |
| GeneID | 655 |
| Source | 重組人骨形態發生蛋白7蛋白Escherichia coli. |
| Molecular Weight | Approximately 15.7 kDa, a monomeric, non-glycosylated polypeptide chain containing 139 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | 重組人骨形態發生蛋白7蛋白STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data is not available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA. |
| Endotoxin | Less than 1 EU/μg of rHuBMP-7 as determined by LAL method. |
| Applications |
1. Molecular standard (Western, ELISA) in studying secreted BMP-7; 2. Preparing antibodies for BMP-7 monomer; 3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE. |
| Reconstitution | 重組人骨形態發生蛋白7蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人骨形態發生蛋白7蛋白 | |
| SDS-PAGE |
|
| Reference |
1. Zeisberg M, Hanai J, Sugimoto H, et al. 2003. Nat Med, 9: 964-8. 2. Phillips FM, Turner AS, Seim HB, 3rd, et al. 2006. Spine J, 6: 500-6. 3. Veerasamy M, Nguyen TQ, Motazed R, et al. 2009. Am J Physiol Renal Physiol, 297: F1238-48. 4. Pauly S, Klatte F, Strobel C, et al. 2012. J Shoulder Elbow Surg, 21: 464-73. |
| Background | Bone Morphogenetic Protein 7 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-7 is mainly expressed in kidney and bladder. It is also present in developing eyes, brain and ear during embryogenesis. BMP-7 also named osteogenic protein-1 (OP-1) is a potent osteoinductive cytokine and plays role in osteoblast differentiation, SMAD1 production and renal development and repair. Human BMP-7 is synthesized with a signal sequence (29 a.a.), a propeptide (263 a.a.), and a growth factor domain (139 a.a.). The growth factor domain of human BMP-7 shares 98 % a.a. sequence identity with mouse and rat BMP-7. |
