產品詳情
簡單介紹：
重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白與其它公司提供的重組蛋白不同，rHuMIP-1β/CCL4蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白
| Synonyms | G-26 T-lymphocyte-secreted protein, HC21, LAG-1, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2 |
| Species | Human |
| Accession | P13236 |
| GeneID | 6351 |
| Source | 重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白Escherichia coli. |
| Molecular Weight | Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuMIP-1β/CCL4 as determined by LAL method. |
| Reconstitution | 重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| 重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白 | |
| Reference |
1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70. 2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5. 3. Garlisi CG, Xiao H, Tian F, et al. 1999. Eur J Immunol. 29:3210-5. 4. Guan E, Wang J, Roderiquez G, et al. 2002. J Biol Chem. 277:32348-52. |
| Background | CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with murine and rat CCL4, respectively. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells. |
