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產品名稱：重組人I-309 / CCL1蛋白
產品型號：rHuI-309/CCL1
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人I-309 / CCL1蛋白與其它公司提供的重組蛋白不同，rHuI-309/CCL1蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人I-309 / CCL1蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人I-309 / CCL1蛋白

Synonyms Small-inducible cytokine A1, T lymphocyte-secreted protein I-309

Species Human

Accession P22362

GeneID 6346

Source Escherichia coli.

Molecular Weight 重組人I-309 / CCL1蛋白Approximately 8.6 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK

Purity >97% by SDS-PAGE and HPLC analyses.

Biological Activity 重組人I-309 / CCL1蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuI-309/CCL1 as determined by LAL method.

Reconstitution 重組人I-309 / CCL1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人I-309 / CCL1蛋白

SDS-PAGE

Reference 1. Miller MD, Krangel MS. 1992. Proc Natl Acad Sci U S A. 89:2950-4.
2. Roos RS, Loetscher M, Legler DF, et al. 1997. J Biol Chem. 272:17251-4.
3. Miller MD, Wilson SD, Dorf ME, et al. 1990. J Immunol. 145:2737-44.

Background Chemokine (C-C motif) ligand 1 (CCL1) belongs to a family inflammatory cytokines and also known as chemokines. It is a small glycoprotein secreted by activated T cells with a molecular weight of approximately 8.5 kDa. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42 % amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines.

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Synonyms	Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
Species	Human
Accession	P22362
GeneID	6346
Source	Escherichia coli.
Molecular Weight	重組人I-309 / CCL1蛋白Approximately 8.6 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK
Purity	>97% by SDS-PAGE and HPLC analyses.
Biological Activity	重組人I-309 / CCL1蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuI-309/CCL1 as determined by LAL method.
Reconstitution	重組人I-309 / CCL1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人I-309 / CCL1蛋白
SDS-PAGE
Reference	1. Miller MD, Krangel MS. 1992. Proc Natl Acad Sci U S A. 89:2950-4. 2. Roos RS, Loetscher M, Legler DF, et al. 1997. J Biol Chem. 272:17251-4. 3. Miller MD, Wilson SD, Dorf ME, et al. 1990. J Immunol. 145:2737-44.
Background	Chemokine (C-C motif) ligand 1 (CCL1) belongs to a family inflammatory cytokines and also known as chemokines. It is a small glycoprotein secreted by activated T cells with a molecular weight of approximately 8.5 kDa. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42 % amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines.

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產品名稱：重組人I-309 / CCL1蛋白