產品詳情
簡單介紹：
重組人**細胞趨化因子/ XCL1蛋白與其它公司提供的重組蛋白不同，rHuLymphotactin/XCL1蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人**細胞趨化因子/ XCL1蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人**細胞趨化因子/ XCL1蛋白
| Synonyms | ATAC, C motif chemokine 1, Cytokine SCM-1, Lymphotaxin, SCM-1-alpha, Small-inducible cytokine C1, XC chemokine ligand 1 |
| Species | Human |
| Accession | P47992 |
| GeneID | 6375 |
| Source | 重組人**細胞趨化因子/ XCL1蛋白Escherichia coli. |
| Molecular Weight | Approximately 10.2 kDa, a single non-glycosylated polypeptide chain containing 92 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人**細胞趨化因子/ XCL1蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuLymphotactin/XCL1 as determined by LAL method. |
| Reconstitution | 重組人**細胞趨化因子/ XCL1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人**細胞趨化因子/ XCL1蛋白 | |
| Reference |
1. Kennedy J, Kelner GS, Kleyensteuber S, et al. 1995. J Immunol. 155:203-9. 2. Yoshida T, Imai T, Takagi S, et al. 1996. FEBS Lett. 395:82-8. 3. Yoshida T, Imai T, Kakizaki M, et al. 1998. J Biol Chem. 273:16551-4. |
| Background | Chemokine (C motif) ligand (XCL1), as known as lymphotactin, is the only known member of the C-chemokine family and signals through the receptor XCR1, formally known as GPR5. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces its chemotactic function by binding to a chemokine receptor called XCR1. Recombinant Human XCL1 which is a single non-glycosylated polypeptide chains containing 92 amino acids and it shares approximately 60 % amino acid sequence homology with the murine and rat protein. |
