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  • 產品名稱:重組人類BCA-1 / CXCL13蛋白

  • 產品型號:rHuBCA-1/CXCL13
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組人類BCA-1 / CXCL13蛋白與其它公司提供的重組蛋白不同,rHuBCA-1/CXCL13蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類BCA-1 / CXCL13蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組人類BCA-1 / CXCL13蛋白

Synonyms Angie, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13
Species Human
Accession O43927
GeneID 10563
Source Escherichia coli.
Molecular Weight 重組人類BCA-1 / CXCL13蛋白Approximately 10.3 kDa, a single non-glycosylated polypeptide chain containing 87 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/ml.
Physical Appearance 重組人類BCA-1 / CXCL13蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Endotoxin Less than 1 EU/μg of rHuBCA-1/CXCL13 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage 重組人類BCA-1 / CXCL13蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類BCA-1 / CXCL13蛋白
Reference 1. Legler DF, Loetscher M, Roos RS, et al. 1998. J Exp Med. 187:655-60.
2. Gunn MD, Ngo VN, Ansel KM, et al. 1998. Nature. 391:799-803.
3. Ansel KM, Harris RB, Cyster JG. 2002. Immunity. 16:67-76.
4. Ansel KM, Ngo VN, Hyman PL, et al. 2000. Nature. 406:309-14.
Background C-X-C motif chemokine 13 encoded by the CXCL13 gene located on human chromosome 4 is constitutively expressed in secondary lymphoid organs. It is also known as B lymphocyte chemoattractant (BLC). As its name suggests, CXCL13 is selectively chemotactic for B cells belonging to both the B-1 and B-2 subsets, and elicits its effects by interacting with chemokine receptor CXCR5. It and its receptor CXCR5 control the organization of B cells within follicles of lymphoid tissues. Recombinant human CXCL13 is a single non-glycosylated polypeptide chain containing 87 amino acids and mature human BCA-1 shares 64 % amino acid sequence similarity with the murine protein and 23–34 % amino acid sequence identity with other known CXC chemokines.

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