產品詳情
簡單介紹：
重組人Macrophage-Derived趨化因子/ CCL22蛋白與其它公司提供的重組蛋白不同，rHuMDC/CCL22蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人Macrophage-Derived趨化因子/ CCL22蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人Macrophage-Derived趨化因子/ CCL22蛋白
| Synonyms | STCP-1, MDC (1-69), Macrophage-derived Chemokine |
| Species | Human |
| Accession | O00626 |
| GeneID | 6367 |
| Source | 重組人Macrophage-Derived趨化因子/ CCL22蛋白Escherichia coli. |
| Molecular Weight | Approximately 8.1 kDa, a single, non-glycosylated polypeptide chain containing 69 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人Macrophage-Derived趨化因子/ CCL22蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 500 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuMDC/CCL22 as determined by LAL method. |
| Reconstitution | 重組人Macrophage-Derived趨化因子/ CCL22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人Macrophage-Derived趨化因子/ CCL22蛋白 | |
| SDS-PAGE |
|
| Reference |
1. Nomiyama H, Imai T, Kusuda J, et al. 1998. Cytogenet Cell Genet, 81: 10-1. 2. Godiska R, Chantry D, Raport CJ, et al. 1997. J Exp Med, 185: 1595-604. 3. Yamashita UandKuroda E. 2002. Crit Rev Immunol, 22: 105-14. 4. Katou F, Ohtani H, Nakayama T, et al. 2001. Am J Pathol, 158: 1263-70. |
| Background | CCL22 is a protein that in humans is encoded by the CCL22 gene, which locates on the Chr. 16. The protein is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69). |
