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產品名稱：重組人Interleukin-20蛋白
產品型號：rHuIL-20
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人Interleukin-20蛋白與其它公司提供的重組蛋白不同，rHuIL-20蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人Interleukin-20蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人Interleukin-20蛋白

Synonyms Cytokine Zcyto10

Species Human

Accession Q9NYY1

GeneID 50604

Source 重組人Interleukin-20蛋白Escherichia coli.

Molecular Weight Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人Interleukin-20蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2, with trehalose.

Endotoxin Less than 1 EU/μg of rHuIL-20 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人Interleukin-20蛋白

SDS-PAGE

Reference 重組人Interleukin-20蛋白1. Sabat R. 2010. Cytokine Growth Factor Rev, 21: 315-24.
2. Blumberg H, Conklin D, Xu WF, et al. 2001. Cell, 104: 9-19.
3. Wei CC, Hsu YH, Li HH, et al. 2006. J Biomed Sci, 13: 601-12.
4. Hsu YH, Li HH, Hsieh MY, et al. 2006. Arthritis Rheum, 54: 2722-33.

Background Human Interleukin-20 (IL-20) is encoded by the IL20 gene located on the chromosome 1 and belongs to the IL-10 family that including IL-10, IL-19, IL-22, IL-24, and IL-26. It is secreted by activated keratinocytes and monocytes, and signals through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 has functions of regulating proliferation and differentiation of keratinocytes during inflammation, and causing cell expansion of multipotential hematopoietic progenitor cells.

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Synonyms	Cytokine Zcyto10
Species	Human
Accession	Q9NYY1
GeneID	50604
Source	重組人Interleukin-20蛋白Escherichia coli.
Molecular Weight	Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人Interleukin-20蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2, with trehalose.
Endotoxin	Less than 1 EU/μg of rHuIL-20 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人Interleukin-20蛋白
SDS-PAGE
Reference	重組人Interleukin-20蛋白1. Sabat R. 2010. Cytokine Growth Factor Rev, 21: 315-24. 2. Blumberg H, Conklin D, Xu WF, et al. 2001. Cell, 104: 9-19. 3. Wei CC, Hsu YH, Li HH, et al. 2006. J Biomed Sci, 13: 601-12. 4. Hsu YH, Li HH, Hsieh MY, et al. 2006. Arthritis Rheum, 54: 2722-33.
Background	Human Interleukin-20 (IL-20) is encoded by the IL20 gene located on the chromosome 1 and belongs to the IL-10 family that including IL-10, IL-19, IL-22, IL-24, and IL-26. It is secreted by activated keratinocytes and monocytes, and signals through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 has functions of regulating proliferation and differentiation of keratinocytes during inflammation, and causing cell expansion of multipotential hematopoietic progenitor cells.

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產品名稱：重組人Interleukin-20蛋白