產(chǎn)品詳情
簡單介紹：
重組體人Interleukin-4蛋白與其它公司提供的重組蛋白不同，rHuIL-4蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組體人Interleukin-4蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組體人Interleukin-4蛋白
| Synonyms | BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra. |
| Species | Human |
| Accession | P05112 |
| GeneID | 3565 |
| Source | Escherichia coli. |
| Molecular Weight | 重組體人Interleukin-4蛋白Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組體人Interleukin-4蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1.0 EU/μg of rHuIL-4 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
|
|
重組體人Interleukin-4蛋白 |
| SDS-PAGE |
|
| Reference |
重組體人Interleukin-4蛋白1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8. 2. Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5. 3. Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8. 4. Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772. 5. Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6. |
| Background | Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. In addition, It enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The human IL-4 has a compact, globular fold, stabilised by 3 disulphide bonds. The human IL-4 shares about 40% aa sequence identity with mouse/rat IL-4 and they are species-specific in their activities. |
