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  • 產品名稱:人角質形成細胞生長因子-2 / FGF-10重組蛋白

  • 產品型號:rHuKGF-2/FGF-10
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
人角質形成細胞生長因子-2 / FGF-10重組蛋白與其它公司提供的重組蛋白不同,-2 / FGF-10重組蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.人角質形成細胞生長因子-2 / FGF-10重組蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

人角質形成細胞生長因子-2 / FGF-10重組蛋白

Species Human
Accession O15520
GeneID 2255
Source Escherichia coli.
Molecular Weight Approximately 19.1 kDa, a single, non-glycosylated polypeptide chain containing 169 amino acids.
Quantity 5μg/25μg/1000μg
AA Sequence LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
Purity 人角質形成細胞生長因子-2 / FGF-10重組蛋白> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 人角質形成細胞生長因子-2 / FGF-10重組蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin Less than 1 EU/μg of rHuKGF-2/FGF-10 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

人角質形成細胞生長因子-2 / FGF-10重組蛋白
SDS-PAGE
Reference 1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.
2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.
3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.
4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.
5. Entesarian M, Matsson H, Klar J, et al. 2005. Nat Genet. 37:125-7.
Background 人角質形成細胞生長因子-2 / FGF-10重組蛋白Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family which is involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant human FGF-10 contains a 208 amino acids and it shares 92 % and 95 % amino acid sequence identity with murine and rat FGF-10. Defects in FGF-10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands and lacrimo-auriculo-dento-digital syndrome.


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