產品詳情
簡單介紹：
重組犬白細胞介素-8 / CXCL8蛋白與其它公司提供的重組蛋白不同，rCaIL-8/CXCL8蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組犬白細胞介素-8 / CXCL8蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組犬白細胞介素-8 / CXCL8蛋白
| Synonyms | (Ser-IL-8)72, GCP/IL-8 protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF |
| Species | Canine |
| Accession | P41324 |
| GeneID | 403850 |
| Source | Escherichia coli. |
| Molecular Weight | 重組犬白細胞介素-8 / CXCL8蛋白Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 79 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | AVLSRVSSEL RCQCIKTHST PFHPKYIKEL RVIDSGPHCE NSEIIVKLFN GNEVCLDPKE KWVQKVVQIF LKKAEKQDP |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組犬白細胞介素-8 / CXCL8蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.15-0.75 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rCaIL-8/CXCL8 as determined by LAL method. |
| Reconstitution | 重組犬白細胞介素-8 / CXCL8蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組犬白細胞介素-8 / CXCL8蛋白 | |
| SDS-PAGE |
|
| Reference |
1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7. 2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62. 3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6. 4. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8. |
| Background | Interleukin-8 (IL-8) is encoded by the IL8 gene andproduced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. . IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis. |
