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產品詳情
  • 產品名稱:重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白

  • 產品型號:rRtEotaxin/CCL11
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白與其它公司提供的重組蛋白不同,rRtEotaxin/CCL11蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白

Synonyms Small-inducible Cytokine A11
Species Rat
Accession P97545
GeneID 29397
Source Escherichia coli.
Molecular Weight 重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
Purity > 96 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin 重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Less than 1 EU/μg of rRtEotaxin/CCL11 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白
SDS-PAGE
Reference 1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12.
2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7.
3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56.
4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5.
Background CCL11 encoded by the gene CCL11is belonging to the CC chemokine family. It is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. CCL11 is a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. CCR3 has been identified to be a specific CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.

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