產(chǎn)品詳情
簡(jiǎn)單介紹：
重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白與其它公司提供的重組蛋白不同，rMuFGF-17蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白
| Species | Murine |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 194 amino acids. |
| AA Sequence | TQGENHPSPN FNQYVRDQGA MTDQLSRRQI REYQLYSRTS GKHVQVTGRR ISATAEDGNK FAKLIVETDT FGSRVRIKGA ESEKYICMNK RGKLIGKPSG KSKDCVFTEI VLENNYTAFQ NARHEGWFMA FTRQGRPRQA SRSRQNQREA HFIKRLYQGQ LPFPNHAERQ KQFEFVGSAP TRRTKRTRRP QSQT |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg in the presence of 10 μg/ml of heparin. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 700 mM NaCl, with 0.02 % Tween-20. |
| Endotoxin | Less than 0.1 EU/μg of rMuFGF-17 as determined by LAL method. |
| Reconstitution | 重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白 | |
| Background | FGF-17 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. The mRNA of FGF-17 was found in midgestation of embryo and multiple ***** tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. |
