產品詳情
簡單介紹：
重組人白細胞介素-36α，158a.a.蛋白與其它公司提供的重組蛋白不同，rHuIL-36α, 158a.a.蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細胞介素-36α，158a.a.蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人白細胞介素-36α，158a.a.蛋白
| Synonyms | FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1 |
| Species | Human |
| Accession | Q9UHA7 |
| GeneID | 27179 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人白細胞介素-36α，158a.a.蛋白Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人白細胞介素-36α，158a.a.蛋白Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rHuIL-36α at 1 μg/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 μg/mL. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuIL-36α, 158a.a. as determined by LAL method. |
| Reconstitution | 重組人白細胞介素-36α，158a.a.蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at - 20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人白細胞介素-36α，158a.a.蛋白 | |
| Reference |
1. Nicklin MJ, Barton JL, Nguyen M, et al. 2002. Genomics. 79:718-25. 2. Dinarello C, Arend W, Sims J, et al. 2010. Nat Immunol. 11:973. 3. Barksby HE, Lea SR, Preshaw PM, et al. 2007. Clin Exp Immunol. 149:217-25. 4. Smith DE, Renshaw BR, Ketchem RR, et al. 2000. J Biol Chem. 275:1169-75. 5. Dunn E, Sims JE, Nicklin MJ, et al. 2001. Trends Immunol. 22:533-6. |
| Background | Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant human interleukin-36 alpha contains 158 amino acids residues which is a single non-glycosylated polypeptide and it is 30 % a.a. identical to IL-1ra, and 27 %, 31 %, 36 %, 46 %, 57% and 28 % a.a. identical to IL-1β, IL-36Ra/IL-1F5, IL-37/IL-1F7, IL-36β/IL-1F8, IL-36γ/IL-1F9 and IL-1F10. |
