產(chǎn)品詳情
簡單介紹：
重組人泛素偶聯(lián)酶E2 C，His蛋白與其它公司提供的重組蛋白不同，rHuUBE2C, His蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人泛素偶聯(lián)酶E2 C，His蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人泛素偶聯(lián)酶E2 C，His蛋白
| Synonyms | UbcH10, Ubiquitin Carrier Protein C, Ubiquitin-protein Ligase C |
| Species | Human |
| Source | Escherichia coli. |
| Molecular Weight | 重組人泛素偶聯(lián)酶E2 C，His蛋白Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids (a.a.) of human UBE2C/UBCH10 and 12 a.a. vector sequence including 6 × His tag at N-terminus. |
| Quantity | 10μg/50μg/1000μg |
| AA Sequence | 重組人泛素偶聯(lián)酶E2 C，His蛋白MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE P |
| Concentration | See label. |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data is not available. |
| Physical Appearance | 重組人泛素偶聯(lián)酶E2 C，His蛋白Sterile Colorless liquid. |
| Formulation | A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 8.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT. |
| Endotoxin | Less than 1 EU/μg of rHuUBE2C, His as determined by LAL method. |
| Storage | Store at -20 °C. |
| 重組人泛素偶聯(lián)酶E2 C，His蛋白 | |
| SDS-PAGE |
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| Reference |
1. Takahashi Y, Ishii Y, Nishida Y, et al. 2006. Cancer Genet Cytogenet, 168: 30-5. 2. Bavi P, Uddin S, Ahmed M, et al. 2011. Am J Pathol, 178: 2109-20. 3. Chen Z, Zhang C, Wu D, et al. 2011. EMBO J, 30: 2405-19. 4. Hao Z, Zhang H, Cowell J. 2012. Tumour Biol, 33: 723-30. |
| Background | Ubiquitin Conjugating Enzyme E2 C belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2C gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBE2C catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis. This activity is essential at the end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBCH10 bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBCH10 is useful for in vitro ubiquitinylation reactions. |
