產品詳情
簡單介紹：
重組人泛素偶聯(lián)酶E2 D3，His蛋白與其它公司提供的重組蛋白不同，rHuUBE2D3, His蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人泛素偶聯(lián)酶E2 D3，His蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人泛素偶聯(lián)酶E2 D3，His蛋白
| Synonyms | Ubiquitin Carrier Protein D3, Ubiquitin-protein Ligase D3 |
| Species | Human |
| Accession | P61077 |
| GeneID | 7323 |
| Source | 重組人泛素偶聯(lián)酶E2 D3，His蛋白Escherichia coli. |
| Molecular Weight | Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids (a.a.) of human UBE2D3/UBC5C and 8 a.a. vector sequence including 6 × His tag at N-terminus. |
| Quantity | 10μg/50μg/1000μg |
| AA Sequence | 重組人泛素偶聯(lián)酶E2 D3，His蛋白MHHHHHHAMA LKRINKELSD LARDPPAQCS AGPVGDDMFH WQATIMGPND SPYQGGVFFL TIHFPTDYPF KPPKVAFTTR IYHPNINSNG SICLDILRSQ WSPALTISKV LLSICSLLCD PNPDDPLVPE IARIYKTDRD KYNRISREWT QKYAM |
| Concentration | See label. |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data is not available. |
| Physical Appearance | Sterile Colorless liquid. |
| Formulation | 重組人泛素偶聯(lián)酶E2 D3，His蛋白A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 6.5, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT. |
| Endotoxin | Less than 1 EU/μg of rHuUBE2D3/UBC5C, His as determined by LAL method. |
| Storage | Store at -20 °C. |
| 重組人泛素偶聯(lián)酶E2 D3，His蛋白 | |
| Reference |
1. Jean SandMoss T. 2008. Differentiation, 76: 431-41. 2. Hattori H, Zhang X, Jia Y, et al. 2007. Blood, 110: 640-50. |
| Background | Ubiquitin-conjugating enzyme E2 D3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2D3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2D3 is a human homolog of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. It mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBα, p105) and abnormal proteins and has 88% and 89% sequence identity with UbcH5a and UbcH5b respectively. |
