產(chǎn)品詳情
簡單介紹：
重組人可溶性CD40配體/ TNFSF5蛋白與其它公司提供的重組蛋白不同，rHusCD40 Ligand/TNFSF5蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人可溶性CD40配體/ TNFSF5蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人可溶性CD40配體/ TNFSF5蛋白
| Synonyms | TNFSF5, sCD40L, CD154, gp39, T-BAM, TNF-related Activation Protein, TRAP |
| Species | Human |
| Accession | P29965 |
| GeneID | 959 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人可溶性CD40配體/ TNFSF5蛋白Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids. |
| Quantity | 10μg/50μg/1000μg |
| AA Sequence | MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人可溶性CD40配體/ TNFSF5蛋白Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
| Endotoxin | Less than 1 EU/μg of rHusCD40L as determined by LAL method. |
| Reconstitution | 重組人可溶性CD40配體/ TNFSF5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人可溶性CD40配體/ TNFSF5蛋白 | |
| Reference |
1. Li R, Chen WC, Pang XQ, et al. 2011. Mol Biol Rep, 38: 5459-64. 2. Reinboldt S, Wenzel F, Rauch BH, et al. 2009. Platelets, 20: 441-4. 3. Tousoulis D, Antoniades C, Nikolopoulou A, et al. 2007. Eur J Clin Invest, 37: 623-8. 4. Varo N, Libby P, Nuzzo R, et al. 2005. Diab Vasc Dis Res, 2: 81-7. 5. Holzer G, Pfandlsteiner T, Blahovec H, et al. 2003. Wien Med Wochenschr, 153: 40-2. |
| Background | CD40 ligand is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family and can be cleaved into two chains: the CD40 ligand membrane form and CD40 ligand soluble form. It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. It mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation. |
