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  • 產品名稱:重組人白細胞介素-9蛋白

  • 產品型號:rHuIL-9
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組人白細胞介素-9蛋白與其它公司提供的重組蛋白不同,rHuIL-9蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細胞介素-9蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組人白細胞介素-9蛋白

Synonyms Cytokine P40, T-cell Growth Factor P40
Species Human
Accession P15248
GeneID 3578
Source Escherichia coli.
Molecular Weight 重組人白細胞介素-9蛋白Approximately 14.1 kDa, a single non-glycosylated polypeptide chain containing 126 amino acids.
Quantity 2μg/10μg/1000μg
AA Sequence QGCPTLAGIL DINFLINKMQ EDPASKCHCS ANVTSCLCLG IPSDNCTRPC FSERLSQMTN TTMQTRYPLI FSRVKKSVEV LKNNKCPYFS CEQPCNQTTA GNALTFLKSL LEIFQKEKMR GMRGKI
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組人白細胞介素-9蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin 重組人白細胞介素-9蛋白Less than 1 EU/μg of rHuIL-9 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人白細胞介素-9蛋白
SDS-PAGE
Reference 1. Renauld JC, Goethals A, Houssiau F, et al. 1990. J Immunol. 144:4235-41.
2. Kelleher K, Bean K, Clark SC, et al. 1991. Blood. 77:1436-41.
Background Interleukin-9 (IL-9) is encoded by the IL9 gene and produced by T-cells and specifically by CD4+ helper cells. IL-9 was originally identified as a cytokine found in the conditioned medium of a human T cell leukemia virus type I (HTLVI) transformed T cell line. It functions through the IL-9 receptor, which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. IL-9 can support the growth of IL-2 independent and IL-4 independent helper T-cells. Human IL-9 has approximately 56 % amino acid sequence identity with murine IL-9. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyper-responsiveness.

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