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產品詳情
  • 產品名稱:重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白

  • 產品型號:rHuHCC-1, 66a.a./CCL14
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白與其它公司提供的重組蛋白不同,rHuHCC-1, 66a.a./CCL14蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白

Synonyms C-C Motif Chemokine 14, HCC-3, NCC-2, Small-inducible Cytokine A14
Species Human
Accession Q16627
GeneID 6358
Source 重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白Escherichia coli.
Molecular Weight Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 66 amino acids.
Quantity 2μg/10μg/1000μg
AA Sequence GPYHPSECCF TYTTYKIPRQ RIMDYYETNS QCSKPGIVFI TKRGHSVCTN PSDKWVQDYI KDMKEN
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-20 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 5 % trehalose.
Endotoxin Less than 1 EU/μg of rHuHCC-1, 66a.a./CCL14 as determined by LAL method.
Reconstitution 重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白
Reference 1. Schulz-Knappe P, Magert HJ, Dewald B, et al. 1996. J Exp Med. 183:295-9.
2. Detheux M, Standker L, Vakili J, et al. 2000. J Exp Med. 192:1501-8.
Background Human CCL14 is belonging to the CC chemokine family. It is encoded by the gene CCL14. CCL14 has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. Recombinant human CCL14 (66 a.a.) contains 66 amino acid residues and activation of the HCC 1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin.

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