產品詳情
簡單介紹：
重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白與其它公司提供的重組蛋白不同，rHuHCC-1, 66a.a./CCL14蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白
| Synonyms | C-C Motif Chemokine 14, HCC-3, NCC-2, Small-inducible Cytokine A14 |
| Species | Human |
| Accession | Q16627 |
| GeneID | 6358 |
| Source | 重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白Escherichia coli. |
| Molecular Weight | Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 66 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | GPYHPSECCF TYTTYKIPRQ RIMDYYETNS QCSKPGIVFI TKRGHSVCTN PSDKWVQDYI KDMKEN |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-20 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 5 % trehalose. |
| Endotoxin | Less than 1 EU/μg of rHuHCC-1, 66a.a./CCL14 as determined by LAL method. |
| Reconstitution | 重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人血液濾液CC趨化因子-1,66a.a./CCL14蛋白 | |
| Reference |
1. Schulz-Knappe P, Magert HJ, Dewald B, et al. 1996. J Exp Med. 183:295-9. 2. Detheux M, Standker L, Vakili J, et al. 2000. J Exp Med. 192:1501-8. |
| Background | Human CCL14 is belonging to the CC chemokine family. It is encoded by the gene CCL14. CCL14 has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. Recombinant human CCL14 (66 a.a.) contains 66 amino acid residues and activation of the HCC 1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin. |
