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產品詳情
  • 產品名稱:重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白

  • 產品型號:rHuMIP-3/CCL23
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白與其它公司提供的重組蛋白不同,rHuMIP-3/CCL23蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白

Synonyms CKB-8, MIP-3, MIPIF-1
Species Human
Accession P55773
GeneID 6368
Source Escherichia coli.
Molecular Weight 重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白Approximately 11.4 kDa, a single, non-glycosylated polypeptide chain containing 99 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin Less than 1 EU/μg of rHuMIP-3/CCL23 as determined by LAL method.
Reconstitution 重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白
SDS-PAGE
Reference 1. Novak H, Muller A, Harrer N, et al. 2007. J Immunol, 178: 4335-41.
2. Votta BJ, White JR, Dodds RA, et al. 2000. J Cell Physiol, 183: 196-207.
3. Poposki JA, Uzzaman A, Nagarkar DR, et al. 2011. J Allergy Clin Immunol, 128: 73-81 e4.
Background CCL23 is a member of CC chemokine family and is encoded by CCL23 gene located on Chr.17 in humans, where near several other CC chemokines. Highly expressed in ***** lung, liver, skeletal muscle and pancreas, this protein shows strong chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. It elicits the effects by binding to CCR1. CCL23 is reported that it can be cleaved into four forms: CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99).

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