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  • 產品名稱:重組人Interleukin-16蛋白

  • 產品型號:rHuIL-16
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組人Interleukin-16蛋白與其它公司提供的重組蛋白不同,rHuIL-16蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人Interleukin-16蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組人Interleukin-16蛋白

Synonyms Interleukin-16 isoform 2, Lymphocyte chemoattractant factor, LCF
Species Human
Accession Q14005
GeneID 3603
Source 重組人Interleukin-16蛋白Escherichia coli.
Molecular Weight Approximately 13.4 kDa, a single non-glycosylated polypeptide chain containing 130amino acids.
Quantity 2μg/10μg/1000μg
AA Sequence 重組人Interleukin-16蛋白MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CD4+ T Lymphocytes is in a concentration range of 1.0-100 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 重組人Interleukin-16蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 7.0.
Endotoxin Less than 1 EU/μg of rHuIL-16, 130 a.a. as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人Interleukin-16蛋白
SDS-PAGE
Reference 1. Baier M, Bannert N, Werner A, et al. 1997. Proc Natl Acad Sci U S A, 94: 5273-7.
2. Zhang Y, Center DM, Wu DM, et al. 1998. J Biol Chem, 273: 1144-9.
3. Cruikshank WW, Kornfeld H, Center DM. 2000. J Leukoc Biol, 67: 757-66.
4. Cruikshank WW, Center DM, Nisar N, et al. 1994. Proc Natl Acad Sci U S A, 91: 5109-13.
5. Mathy NL, Scheuer W, Lanzendorfer M, et al. 2000. Immunology, 100: 63-9.
Background Human Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it has three isoforms which are cleaved from pre-Interleukin-16. IL-16 is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant human IL-16 (130 a.a.) contains 130 amino acid residues and it shares 85 % a.a. sequence identity with murine IL-16.

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