產(chǎn)品詳情
簡單介紹：
重組人中腦的Astrocyte-Derived神經(jīng)營養(yǎng)因子蛋白與其它公司提供的重組蛋白不同，rHuMANF蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人中腦的Astrocyte-Derived神經(jīng)營養(yǎng)因子蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人中腦的Astrocyte-Derived神經(jīng)營養(yǎng)因子蛋白
| Synonyms | ARMET |
| Species | Human |
| Accession | P55145 |
| GeneID | 7873 |
| Source | 重組人中腦的Astrocyte-Derived神經(jīng)營養(yǎng)因子蛋白Escherichia coli. |
| Molecular Weight | Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing158 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人中腦的Astrocyte-Derived神經(jīng)營養(yǎng)因子蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuMANF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組人中腦的Astrocyte-Derived神經(jīng)營養(yǎng)因子蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人中腦的Astrocyte-Derived神經(jīng)營養(yǎng)因子蛋白 | |
| SDS-PAGE |
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| Reference |
1. Petrova P, Raibekas A, Pevsner J, et al. 2003. J Mol Neurosci. 20:173-88. 2. Airavaara M, Chiocco MJ, Howard DB, et al. 2010. Exp Neurol. 225:104-13. 3. Lindholm P, Peranen J, Andressoo JO, et al. 2008. Mol Cell Neurosci. 39:356-71. |
| Background | MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Mature human MANF is 99 %, 98 % and 96 % a.a. identical to mature rat, mouse and bovine MANF respectively. |
