Cat. Number:

Anti-Shugoshin抗體KL-17476R

Quantity size:

0.2ml

Concentration:

1mg/ml   Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

Sgo1 is a 561 amino acid nuclear and cytoplasmic protein that is widely expressed with highest expression in testis. Sgo1Anti-Shugoshin抗體 localizes to the inner centromere throughout prophase until metaphase. Sgo1 is suggested to prevent premature dissociation of the cohesin complex from centromeres after prophase by impeding phosphorylation of the SA-2 subunit of the cohesin complex at the centromere. This ensures cohesin persistence at the centromere until cohesin cleavage is achieved by Separase at the anaphase stage of mitosis. Sgo1 is essential for proper chromosome segregation and for proper attachment of spindle microtubule to the kinetochore. Sgo1 may also play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating Plk kinetochore affinity. Sgo1 exists as seven alternatively isoforms one of which (isoform 3) does not does not localize to kinetochores during any stages of the cell cycle

Also known as:

hSgo 1; hSgo1; NY BR 85;Anti-Shugoshin抗體 Serologically defined breast cancer antigen NY BR 85; Serologically defined breast cancer antigen NY-BR-85; SGO 1; SGO; SGO L1; SGO1; SGOL 1; SGOL1; SGOL1_HUMAN; Shugoshin 1AB protein; Shugoshin 1CD protein; Shugoshin 1EFprotein; Shugoshin 1GH protein; Shugoshin 1KL protein; Shugoshin like 1 (S. pombe); Shugoshin like 1; Shugoshin-like 1.

Specificity:

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500   
Not yet tested in other applications. Anti-Shugoshin抗體
Optimal working dilutions must be determined by the end user.